Elisa M. Konieczko
Professor, Biology Department
Office: Z 203
- Courses Taught
- Educational History
- Professional Experience
- Professional Societies
Faculty Advisor and research lab mentor, Gannon University subaward, in collaboration with Penn State Behrend, from the National Aeronautic and Space Association/Pennsylvania Space Grant Consortium. Other faculty advisors: Wookwon Lee, H.Rick Diz, Hwidong Kim, Steve Frezza, Lin Zhao, and Qing Zheng, January 2014-December 2015, $20,000.
Co-PI, National Science Foundation Award HRD-1107015, Advance Grant, TRANSFORM:Teaching Research Advancement Network to Secure Female Faculty for Organizational Retention and Management. Melanie L. Hatch, PI; other Co-PIs: Karinna M. Vernaza,Theresa M. Vitolo, Weslene T. Tallmadge, September 1, 2011-August 31, 2016,$385,919.
Palumbo Trust Foundation Grant The Palumbo Trust Foundation request for $100,000 in Molecular Biology Equipment. Sarah Ewing, Elisa M. Konieczko. March 2012, funded in part, $25,000.
Beta Beta Beta Biological Honors Society and Sigma Xi National Student Grants
Recipient of BBB Biological National Honor Society Grant, with Alyson Fuller, Gannon biology Major, Fall, 1998, funded in full (~$300)
Recipient of BBB Biological National Honor Society Grant, with Scott Lindsay, Gannon biology Major, Fall 2000, funded in full (~$500)
Recipient of Sigma Xi, the Research Society Grant, with Scott Lindsay, Gannon biology Major, Fall 2000, funded in full ($1000)
Recipient of BBB Biological National Honor Society Grant, with Matt Ruston, Gannon biology Major, Fall 2009, funded in full ($850)
Recipient of BBB Biological National Honor Society Grant, with Morgan Thompson, Gannon biology Major, Fall 2014, funded in full ($1329)
Recipient of BBB Biological National Honor Society Grant, with Jessie Milliken, Gannon biology Major, Fall 2014, funded in full ($1159)
Internal Grants at Gannon University
Recipient of Faculty Development Award, Fall 1998; Fall 2000; Fall 2002; Fall 2004; Fall 2006; Fall 2007, Spring 2008, Fall 2008, Fall 2009, Spring 2010, Fall 2010, Fall 2011, Spring 2012, Spring 2013, Fall 2014, Fall 2015
Recipient of Faculty Research Award, Spring 1999, Spring 2001, Spring 2003, Spring 2005, Spring 2011
Duratz Student Research Grants
Recipient of Duratz Undergraduate Student Research Grant, with Andrew Carmisino, Gannon biology Major, Fall 2004, funded in full
Recipient of Duratz Undergraduate Student Research Grant, with Kathryn Billig, Gannon biology Major, Spring 2008, partially funded
Recipient of Duratz Undergraduate Student Research Grant, with Koreen Morgan, Gannon biology Major, Fall 2010, funded in full
Recipient of Duratz Undergraduate Student Research Grant, with Jennifer Connell, Gannon biology Major, Fall 2011, funded in full
Recipient of Gannon University’s Student Government Association Teacher of the Month Award, November 2003
Recipient of Gannon University Undergraduate Research Award for excellence in mentoring undergraduate students in research, Spring 2007
Recipient of Gannon University’s Excellence in Advising Award for the Mororsky College of Health Professions and Sciences, Spring 2012
Recepient, with Dr. Catherine Mattinson, of the Best Electronic Poster Award at the 2016 Center for Teaching and Learning Expo, January 2016
I am a second generation American of Italian and Polish descent. I was raised in Erie, PA but lived in several East Coast cities while completing my dissertation and postdoctoral fellowships. I returned to Erie as an Assistant Professor in the Biology Department at Gannon University and am now a full Professor of Biology. My primary teaching responsibilities are in the areas of cadaver based Human Gross Anatomy and Cell Biology. My research is conducted in collaboration with local physicians and researchers and currently investigates the role of the hormones relaxin and estrogen on human fibroblasts and keratinocytes. Gannon University undergraduate students work in my research lab on these collaborative projects.
In my spare time, I like cooking, making jewelry, crocheting, and reading/watching science fiction.I met my husband, Chet LaPrice, at a Gannon University Christmas party. He is the Operations Manager at Gannon University's radio station, WERG 90.5. Together, we like to travel, cross country ski, bike ride, and attend local and national baseball games.
Human Gross Anatomy (BIOL 365)
Human Gross Anatomy laboratory (BIOL 366)
Cell Biology (BIOL 375)
Cell Biology laboratory (BIOL 376)
Mechanical and Biological Wonders of Panama [includes a Spring Break Trip to Panama] (ENG 201)
Special Topics in Biology (BIOL 492)
Animal Form and Function (BIOL 124)
Essentials of Anatomy and Physiology I (BIOL 108)
Gannon University, B.S. in Biology, 1984
University of Pittsburgh School of Medicine, Ph.D., Thesis Title: The Interaction of Vesicular Stomatitis Virus with Baby Hamster Kidney Cells and Rat Embryo Fibroblasts, Thesis Advisor: Christopher C. Widnell, Ph.D.; Department of Neurobiology, Anatomy, and Cell Science; Research area: Cell Biology; 1992
Postdoctoral Fellow in the laboratory of James D. Jamieson, M.D., Ph.D.
March, 1992 - May, 1995
Department of Cell Biology
Yale School of Medicine
333 Cedar Street
New Haven, CT 06511
Recipient of Anna Fuller Foundation Fellowship, 3/1992 to 3/1994
Postdoctoral Fellow in the laboratory of James M. Crawford, M.D., Ph.D.
January, 1997- July, 1997
July, 1995 - December, 1996
Department of Pathology
Brigham and Women's Hospital
Department of Pathology
Yale School of Medicine
Assistant Professor, Biology Department, Gannon University, August 1997-July 2002
Associate Professor, Biology Department, Gannon University, August 2002-2011
Tenured, May 2004
Director of Gannon’s “3 + 4" LECOM Accelerated Osteopathic Medical Program
August 1998- June 2006
Director of Gannon’s University Scholars Program
Director of Gannon’s “4 + 4" LECOM Medical Program
September 2002- June 2006
Director of Gannon’s Pre-Pharmacy Programs
July 2004- June 2009
Coordinator of Undergraduate Research
September 2006 – June 2011
Director, Biotechnology Program
July 2008- June 2010
Professor, Biology Department, Gannon University, August 2011-
Vice President, Faculty Senate, 2013-2014
President, Faculty Senate, 2014-2015
American Society for Cell Biology; Education Committee, 2005-2012; Public Information Committee, 2013-
American Association of Anatomists, Public Policy Committee, 2005-2008, Anatomical Nomenclature Committee, 2012-2013
Peer Reviewed Publications:
Konieczko, E.M. "Education Minisymposium: Envisioning Educational Change in the Digital Age." 2014 Annual Meeting Highlights of the American Society for Cell Biology, ASCB Newsletter, January-February 2015 Issue, pp. 14 - 15.
Elisa M. Konieczko and Catherine E. Mattinson. 2014-2016. Gannon University Human Gross Anatomy Lab Dissector. Gannon University Press (Erie, PA).
Vagula, Mary C. and Elisa M. Konieczko. 2012. “Lipid Peroxidation and Polybrominated Diphenyl Ethers-A Toxicological Perspective.” Chapter 24 in Lipid Peroxidation. ISBN 980-953-143-0. InTech (Rejeka, Croatia).
Karinna M. Vernaza and Elisa M. Konieczko. 2010. Better Learning through Collaboration and Assessment: Development of an Interdisciplinary Study Abroad Experience. ASEE 2010 NC Sectional Conference Proceedings. Pittsburgh, PA, March 26-27, 2010. www.asee-nc.org, and via CD.
Konieczko, E.M. 2009. Hello Old Friend, My How You’ve Changed. CBE Life Sciences Education. Vol. 8:167-168.
Cooney TE, Konieczko EM, Roach L, Poole B. Fate of Mu Receptors During Rat Skeletogenesis. Orthopedics 32(2):95, February 2009. (Full article available online at http://www.orthosupersite.com/view.asp?rID=35999).
Cooney TE, Schober JM, Lubahn JD, Konieczko EM. Relaxin's involvement in extracellular matrix homeostasis: two diverse lines of evidence. Ann NY Acad Sci 1160: 329-35, 2009.
Lubahn J, Ivance D, Konieczko E, Cooney T. Immunohistochemical detection of relaxin binding to the volar oblique ligament. J Hand Surg [Am]. 2006 Jan;31(1):80-4.
Sasi S., Konieczko, E.M., Contrafatto I., and J.D. Maloney. 2003. Modeling of Catheter-based Ablation of the left atrial-pulmonary vein junction in human hearts. Proceedings of the International Conference on Mathematics and Engineering Techniques in Medicine and Biological Sciences (METMBS’03), June 23-26, 2003, Las Vegas, Nevada, F. Valafar and H. Valafar, Eds., CSREA Press, pp. 417-423.
Galey S., Konieczko E.M., Arnold C.A., and Cooney T. 2003. Immunohistological detection of relaxin binding to anterior cruciate ligaments. Orthopedics. 26:1201-1204.
Galey S., Lubahn J., Ivance D., Konieczko E., Arnold C., and Cooney T. 2001. Immunohistochemical detection of relaxin binding to ligaments of the thumb and knee. Proceedings of the 3rd International Conference on Relaxin and Related Peptides, October 22-27, 2000 in Broome, Australia. Jeffrey Tregear, Richard Ivell, Ross A. Bathgate, and John D. Wade, Eds, Kluwer Academic Publishers, Boston, pp. 421-424.
Konieczko, E.M., and J. M. Crawford. 1999. Tropic effects of taurocholate on the bile secretory phenotype of WIF-B hepatoma cells. FALK Symposium 108: Bile Acids and Cholestasis. G. Paumgartner, A. Stiehl, W. Gerok, D. Keppler, U. Leuschner, Eds. Kluwer Academic Publishers, Boston, pp.166-171.
Konieczko, E.M., A.K. Ralston, A.C. Crawford, S.J. Karpen, and J.M. Crawford. 1998. Enhanced Na+-dependent bile salt uptake by WIF-B cells, a rat hepatoma hybrid cell line, following growth in the presence of a physiological bile salt. Hepatology, 27 (1):191-199.
Valentijn, J.A., D. Sengupta, F.D. Gumkowski, L.H. Tang, E.M. Konieczko, and J.D. Jamieson. 1996. Rab3D localizes to secretory granules in rat pancreatic acinar cells. Eur. J. Cell Biol. 70:33-41.
Konieczko, E.M., P.A. Whitaker-Dowling, and C.C. Widnell. 1994. Membrane fusion as a determinant of the infectibility of cells by vesicular stomatitis virus. Virology. 199:200-211.
Jena, B.P., F.D. Gumkowski, E.M. Konieczko, G. Fischer von Mollard, R. Jahn, and J.D. Jamieson. 1994. Redistribution of a rab3-like GTP-binding protein from secretory granules to the Golgi complex in pancreatic acinar cells during regulated exocytosis. J. Cell Biol. 124:43-53.
Pasternak, C.A., J.E.J. Aiyathurai, V. Makinde, A. Davies, S.A. Baldwin, E.M. Konieczko, and C.C. Widnell. 1991. Regulation of glucose uptake by stressed cells. J. Cell Physiol. 149:324-331.
My research lab investigates two main projects, both involving the hormones relaxin and estrogen. Relaxin, a peptide hormone traditionally associated with pregnancy, has recently been identified as having an effect on collagen turnover, specifically to inhibit collagen synthesis while simultaneously enhancing collagen breakdown through the activation of matrix metalloproteinases. It is found in both women and men, as is the more familiar steroid hormone estrogen. This hormone is responsible for the secondary sexual characteristics of women and plays a fundamental role in female sexuality and reproduction.
The first project is being done in collaboration with Dr. John Lubahn and Mr. Tim Cooney. A summary of this project: Female athletes injure their anterior cruciate ligament (ACL) up to six times more than male athletes. There are numerous risks for the injury but one of the most controversial risks includes hormonal influences causing structural changes. Estrogen’s role has already been well established in the structure of the collagen of the ACL; while the hormone relaxin which acts on connective tissues of the pubic symphysis, uterus, and cervix to increase the flexibility for successful parturition is another possibility for structural changes. This study also used the volar oblique ligament (VOL) of the hand, which often becomes damaged and non-functional in postmenopausal women. The main goal of this research project was grow cells from tiny pieces of the ligaments, called explants, and to discover the identity of those cells. We have established through immunocytochemical staining that the cells grown from the explants are fibroblasts. Subsequently, this study looked at the localization of relaxin receptors on these fibroblast derived from ACLs or VOLs, and to compare it to the localization of estrogen receptors. Explants were taken from male and female patients ranging from ages 16-67 and immunofluorescent staining was performed on cells generated from the explants. Results showed that both estrogen and relaxin receptors localized to the cultured fibroblasts, with relaxin and estrogen receptors having similar staining patterns. This suggests that both hormones affect ACLs through similar mechanisms. Currently, we are examining the effects of growth in both estrogen and/or relaxin on the structure of the fibroblasts. Two types of experiments will be done in the next few months: time frame experiments (incubation at 24, 48 or 96 hours) and dose response experiments (different concentrations of both hormones).
The second project is being done in collaboration with Dr. Justine Schober and Mr. Tim Cooney. A summary of this project:
One disadvantage of surgical procedures is the formation of aesthetically unpleasant scars resulting from the healing process. Keratinocytes are the cells within the epidermis responsible for scar formation. Previous work from our lab has demonstrated the presence of both relaxin and estrogen receptors on keratinocyte membranes and in the cytosol of these cells4. This study will use primary epidermal keratinocytes derived from normal, human neonatal foreskin. This cell line, commercially available from the American Type Culture Collection (Cat. PCS-200-101) will be grown with or without estrogen and/or relaxin. To examine the effects of chronic exposure to hormones, cells will be grown for seven days in the presence or absence of 20 ug/ml estrogen or 100 pg/ml relaxin (physiological concentrations), and then fixed with formalin and permeabilized with methanol. Cells will also be grown in the absence or presence of both hormones. Fixed and/or permeablized cells will be subsequently incubated with primary antibodies directed against the receptors for relaxin or estrogen, another peptide hormone, and then with secondary antibodies conjugated to rhodamine or fluoresceine. Cells will also be stained with rhodamine-phalloidin to visualize actin, a cytoskeletal marker protein. All immunostained cells will be analyzed using confocal microscopy. We hypothesize that growth in exogenous hormones will down regulate the presence of hormones on the plasma membrane and in the cytosol.
Habitat for Humanity, Erie, PA Affiliate; Family Selection Committee (2009- present) ; Chair, Family Selection Committee (2010 - 2013; 2015 - present); Board Member (2013 - present); Board Secretary (2015-present)
Crime Victim Center, Erie PA, Board Member (2012-2015)