Elisa M. Konieczko

Professor, Biology Department
Faculty Career Mentoring Crd, Academic Affairs
Phone: 814-871-7651
Office: Z 203
konieczk001@gannon.edu

  • Awards/Grants
  • Biography
  • Courses Taught
  • Educational History
  • Professional Experience
  • Professional Societies
  • Publications
  • Scholarship/Research
  • Service

Awards/Grants

 National Grants

Faculty Advisor and research lab mentor, Gannon University subaward, in collaboration with Penn State Behrend,  from the National  Aeronautic and Space Association/Pennsylvania Space Grant Consortium. Other faculty advisors: Wookwon Lee, H.Rick Diz, Hwidong Kim, Steve Frezza, Lin Zhao, and Qing Zheng, January 2014-December 2015, $20,000.

Co-PI, National Science Foundation Award HRD-1107015, Advance Grant, TRANSFORM:Teaching Research Advancement Network to Secure Female Faculty for Organizational Retention and Management.  Melanie L. Hatch, PI; other Co-PIs: Karinna M. Vernaza,Theresa M. Vitolo, Weslene T. Tallmadge, September 1, 2011-August 31, 2016,$385,919.

Palumbo Trust Foundation Grant The Palumbo Trust Foundation request for $100,000 in Molecular Biology Equipment. Sarah Ewing, Elisa M. Konieczko. March 2012, funded in part, $25,000.

Beta Beta Beta Biological Honors Society and Sigma Xi National Student Grants

Recipient of BBB Biological National Honor Society Grant, with Alyson Fuller, Gannon biology Major, Fall, 1998, funded in full (~$300)

Recipient of BBB Biological National Honor Society Grant, with Scott Lindsay, Gannon biology Major, Fall 2000, funded in full (~$500)

Recipient of Sigma Xi, the Research Society Grant, with Scott Lindsay, Gannon biology Major, Fall 2000, funded in full ($1000)

Recipient of BBB Biological National Honor Society Grant, with Matt Ruston, Gannon biology Major, Fall 2009, funded in full ($850)

Recipient of BBB Biological National Honor Society Grant, with Morgan Thompson, Gannon biology Major, Fall 2014, funded in full ($1329)

Recipient of BBB Biological National Honor Society Grant, with Jessie Milliken, Gannon biology Major, Fall 2014, funded in full ($1159

Internal Grants at Gannon University

Recipient of Faculty Development Award, Fall 1998; Fall 2000; Fall 2002; Fall 2004; Fall 2006;   Fall 2007, Spring 2008, Fall 2008, Fall 2009, Spring 2010, Fall 2010, Fall 2011, Spring 2012, Spring 2013, Fall 2014, Fall 2015, Fall 2016, Spring 2017

Recipient of Faculty Research Award, Spring 1999, Spring 2001, Spring 2003, Spring 2005, Spring 2011

Duratz Student Research Grants

Recipient of Duratz Undergraduate Student Research Grant, with Andrew Carmisino, Gannon biology Major, Fall 2004, funded in full

Recipient of Duratz Undergraduate Student Research Grant, with Kathryn Billig, Gannon biology Major, Spring 2008, partially funded

Recipient of Duratz Undergraduate Student Research Grant, with Koreen Morgan, Gannon biology Major, Fall 2010, funded in full

 Recipient of Duratz Undergraduate Student Research Grant, with Jennifer Connell, Gannon biology Major, Fall 2011, funded in full

 Awards

Recipient of Gannon University's Distinguished Faculty Award,  2017

Recipient of Gannon University’s Student Government Association Teacher of the Month Award, November 2003

Recipient of Gannon University Undergraduate Research Award for excellence in mentoring undergraduate students in research, Spring 2007

Recipient of Gannon University’s Excellence in Advising Award for the Mororsky College of Health Professions and Sciences, Spring 2012

Recepient, with Dr. Catherine Mattinson, of the Best Electronic Poster Award at the 2016 Center for Teaching and Learning Expo, January 2016

Biography

I am a second generation American of Italian and Polish descent. I was raised in Erie, PA but lived in several East Coast cities while completing my dissertation and postdoctoral fellowships. I returned to Erie as an Assistant Professor in the Biology Department at Gannon University and am now a full Professor of Biology. My primary teaching responsibilities are in the areas of cadaver based Human Gross Anatomy and Cell Biology. My research is conducted in collaboration with local physicians and researchers and currently investigates the role of the hormones relaxin and estrogen on human fibroblasts and keratinocytes. Gannon University undergraduate students work in my research lab on these collaborative projects.

In my spare time, I like cooking, making jewelry, crocheting, and reading/watching science fiction.I met my husband, Chet LaPrice, at a Gannon University Christmas party. He is the Operations Manager at Gannon University's radio station, WERG 90.5. Together, we like to travel, cross country ski, bike ride, and attend local and national baseball games.

Courses Taught

  • Human Gross Anatomy (BIOL 365)
  • Human Gross Anatomy laboratory (BIOL 366)
  • Cell Biology (BIOL 375)
  • Cell Biology laboratory (BIOL 376)
  • Mechanical and Biological Wonders of Panama [includes a Spring Break Trip to Panama] (ENG 201)
  • Special Topics in Biology (BIOL 492)
  • Animal Form and Function (BIOL 124)
  • Essentials of Anatomy and Physiology I (BIOL 108)

Educational History

  • Gannon University, B.S. in Biology, 1984
  • University of Pittsburgh School of Medicine, Ph.D., Thesis Title: The Interaction of Vesicular Stomatitis Virus with Baby Hamster Kidney Cells and Rat Embryo Fibroblasts,  Thesis Advisor: Christopher C. Widnell, Ph.D.;  Department of Neurobiology, Anatomy, and Cell Science; Research area: Cell Biology; 1992

Professional Experience

  • Postdoctoral Fellow in the laboratory of James D. Jamieson, M.D., Ph.D.
      March, 1992 - May, 1995
      Department of Cell Biology
      Yale School of Medicine
      333 Cedar Street
      New Haven, CT 06511
      Recipient of Anna Fuller Foundation Fellowship, 3/1992 to 3/1994

 

  • Postdoctoral Fellow in the laboratory of James M. Crawford, M.D., Ph.D.
      July, 1995 - December, 1996
      Department of Pathology
      Brigham and Women's Hospital

      January, 1997- July, 1997
      Department of Pathology
      Yale School of Medicine

 

  • Assistant Professor, Biology Department, Gannon University, August 1997-July 2002

  • Associate Professor, Biology Department, Gannon University, August 2002-2011
      Tenured, May 2004
      Director of Gannon’s “3 + 4" LECOM Accelerated Osteopathic Medical Program
       August 1998- June 2006
      Director of Gannon’s University Scholars Program
       August 2000-2003
      Director of Gannon’s “4 + 4" LECOM Medical Program
       September 2002- June 2006
      Director of Gannon’s Pre-Pharmacy Programs
       July 2004- June 2009
      Coordinator of Undergraduate Research
       September 2006 – June 2011
      Director, Biotechnology Program
       July 2008- June 2010

  •  Professor, Biology Department, Gannon University, August 2011-

      Vice President, Faculty Senate, 2013-2014
      President, Faculty Senate, 2014-2015

Professional Societies

  • American Society for Cell Biology; Education Committee, 2005-2012; Public Information Committee, 2013-
  • American Association of Anatomists, Public Policy Committee, 2005-2008, Anatomical Nomenclature Committee, 2012-2013

Publications

Konieczko, E.M. (Producer), Shaw, J. (Executive Producer), Weigert, R., (Director) and members of the Weigert Lab (Actors). (2016). Discovery Inside Living Cells in Multicellular   Organisms [Motion Picture]. United States of America: Celldance Studios 2016 Production;             https://vimeo.com/193753622/2824ddc8d7

Konieczko, E.M.  2016."Sharing Expertise through the MALT Program." ASCB Newsletter, November/December 2016 Issue, Vol 39, Number 8, pgs. 15-16.

Vitolo, T. M., Vernaza, K. M., Lindley, L., Konieczko, E. M., Tallmadge, W., and Sasi, S. 2016. Making Changes: Application of an NSF-ADVANCE PAID Grant to a Predominately Undergraduate Institution (PUI). [PDF] [Powerpoint slides]. Proceedings of the ASEE 123rd Annual Conference & Exposition, June 26 – 29, New Orleans, LA. Washington, D.C.: American Society for Engineering Education (ASEE).Peer Reviewed Publications:

Konieczko, E.M. "Education Minisymposium: Envisioning Educational Change in the Digital Age." 2014 Annual Meeting Highlights of the American Society for Cell Biology,  ASCB Newsletter, January-February 2015 Issue, pp. 14 - 15.

Elisa M. Konieczko and Catherine E. Mattinson. 2014-2016. Gannon University Human Gross Anatomy Lab Dissector. Gannon University Press (Erie, PA).

Vagula, Mary C. and Elisa M. Konieczko. 2012. “Lipid Peroxidation and Polybrominated Diphenyl  Ethers-A Toxicological Perspective.” Chapter 24 in Lipid Peroxidation. ISBN 980-953-143-0.  InTech (Rejeka, Croatia).

Karinna M. Vernaza and Elisa M. Konieczko. 2010. Better Learning through Collaboration and Assessment: Development of an Interdisciplinary Study Abroad Experience.  ASEE 2010 NC Sectional Conference Proceedings. Pittsburgh, PA, March 26-27, 2010.  www.asee-nc.org, and via CD.

Konieczko, E.M. 2009. Hello Old Friend, My How You’ve Changed.  CBE Life Sciences Education. Vol. 8:167-168.

Cooney TE, Konieczko EM, Roach L, Poole B. Fate of Mu Receptors During Rat Skeletogenesis. Orthopedics 32(2):95, February 2009. (Full article available online at http://www.orthosupersite.com/view.asp?rID=35999).

Cooney TE, Schober JM, Lubahn JD, Konieczko EM.  Relaxin's involvement in extracellular matrix homeostasis: two diverse lines of evidence.  Ann NY Acad Sci 1160: 329-35, 2009.

Lubahn J, Ivance D, Konieczko E, Cooney T. Immunohistochemical detection of relaxin binding to the volar oblique ligament. J Hand Surg [Am]. 2006 Jan;31(1):80-4.

Sasi S., Konieczko, E.M., Contrafatto I., and J.D. Maloney. 2003. Modeling of Catheter-based Ablation of the left atrial-pulmonary vein junction in human hearts. Proceedings of the International Conference on Mathematics and Engineering Techniques in Medicine and Biological Sciences (METMBS’03), June 23-26, 2003, Las Vegas, Nevada, F. Valafar and H. Valafar, Eds., CSREA Press, pp. 417-423.

Galey S.,  Konieczko E.M., Arnold C.A., and Cooney T. 2003. Immunohistological detection of relaxin binding to anterior cruciate ligaments. Orthopedics. 26:1201-1204.

Galey S., Lubahn J., Ivance D., Konieczko E., Arnold C., and Cooney T. 2001. Immunohistochemical detection of relaxin binding to ligaments of the thumb and knee. Proceedings of the 3rd International Conference on Relaxin and Related Peptides, October 22-27, 2000 in Broome, Australia.  Jeffrey Tregear, Richard Ivell, Ross A. Bathgate, and John D. Wade, Eds, Kluwer Academic Publishers, Boston, pp. 421-424.

Konieczko, E.M., and J. M. Crawford. 1999. Tropic effects of taurocholate on the bile secretory phenotype of WIF-B hepatoma cells. FALK Symposium 108: Bile Acids and Cholestasis. G. Paumgartner, A. Stiehl, W. Gerok, D. Keppler, U. Leuschner, Eds. Kluwer Academic Publishers, Boston, pp.166-171.

Konieczko, E.M., A.K. Ralston, A.C. Crawford, S.J. Karpen, and J.M. Crawford. 1998. Enhanced Na+-dependent bile salt uptake by WIF-B cells, a rat hepatoma hybrid cell line, following growth in the presence of a physiological bile salt. Hepatology, 27 (1):191-199.

Valentijn, J.A., D. Sengupta, F.D. Gumkowski, L.H. Tang, E.M. Konieczko, and J.D. Jamieson. 1996. Rab3D localizes to secretory granules in rat pancreatic acinar cells. Eur. J. Cell Biol. 70:33-41.

Konieczko, E.M., P.A. Whitaker-Dowling, and C.C. Widnell. 1994.  Membrane fusion as a determinant of the infectibility of cells by vesicular stomatitis virus. Virology. 199:200-211.

Jena, B.P., F.D. Gumkowski, E.M. Konieczko, G. Fischer von Mollard, R. Jahn, and J.D. Jamieson. 1994. Redistribution of a rab3-like GTP-binding protein from secretory granules to the Golgi complex in pancreatic acinar cells during regulated exocytosis.  J. Cell Biol. 124:43-53.  

Pasternak, C.A., J.E.J. Aiyathurai, V. Makinde, A. Davies, S.A. Baldwin, E.M. Konieczko, and C.C. Widnell. 1991. Regulation of glucose uptake by stressed cells. J. Cell Physiol. 149:324-331.

Scholarship/Research

My research lab investigates two main projects,

The first involves the hormones relaxin and estrogen.  Relaxin, a peptide hormone traditionally associated with pregnancy, has recently been identified as having an effect on collagen turnover, specifically to inhibit collagen synthesis while simultaneously enhancing collagen breakdown through the activation of matrix metalloproteinases.  It is found in both women and men, as is the more familiar steroid hormone estrogen.  This hormone is responsible for the secondary sexual characteristics of women and plays a fundamental role in female sexuality and reproduction.         This project is being done in collaboration with Dr. John Lubahn and Mr. Tim Cooney.  This study uses the volar oblique ligament (VOL) of the hand, which often becomes damaged and non-functional in postmenopausal women, and a myofibroblast cell line. The main goal of this research project was grow cells from tiny pieces of the ligaments, called explants, and to discover the identity of those cells.  We have established through immunocytochemical staining that the cells grown from the explants are fibroblasts. Subsequently, this study looked at the localization of relaxin receptors on these fibroblast derived from ACLs or VOLs, and to compare it to the localization of estrogen receptors. Explants were taken from male and female patients ranging from ages 16-67 and immunofluorescent staining was performed on cells generated from the explants. These experiements were also conducted on the myofibroblast cells. Results showed that both estrogen and relaxin receptors localized to the cultured fibroblasts, with relaxin and estrogen receptors having similar staining patterns. This suggests that both hormones affect ligament cells and myofibroblasts through similar mechanisms.  Currently, we are examining the effects of growth in both estrogen and/or relaxin on  nucleic acids (RNA and DNA) extracted from experimental and control cells.  

The second project is done in collaboration with Mr. Zach Murphy, a graduate student in the James Palis Lab at the University of Rochester. The Palis lab investigates the maturation of human red blood cells. During maturation, red blood cells lose their nucleus  before entering the circulatory system. Other cells in the body lose their nucleus in a process called apoptosis, also known as programmed cell death. Apoptosis is normal and required for controlling and regulating the number of cells in an organism. It occurs in both embryos and adult organism.Extracellular or intracellular signals can cause cells to undergo apoptosis. The  Bcl-2 family of proteins plays a major role in regulating apoptosis. After apoptosis is activated, a series of cascade events follow. Binding of a Fas ligand starts an energy dependent, death-inducing process, activating procaspases which in turn starts a cascade that  ultimately leads to the cell’s death. One of the easiest ways to figure out if a cell is undergoing apoptosis is to analyze the morphological changes of cells. During preliminary apoptotic stages, cells undergo shrinkage and pyknosis, before they ultimately fragment. The mitochondria also become active. Another visible change is the blebbing of the plasma membrane.  BHK21 cells are an adherent, immortal cell line derived from the kidneys of one day old Mesocrietus auratus, commonly referred to as Syrian golden hamsters. The basic morphology of BHK21 cells resembles fibroblasts, exhibiting a flat, elongated surface and containing extended  processes along the length of the cell. The nucleus is centrally located in the cell and is flat and oval in shape. This study uses baby hamster kidney (BHK21) cells to study apoptosis. Cells were incubated with one of two apoptotic inducers (HMA or Staurosporine) and then analyzed using multispectral imaging flow cytometry for changes to the nucleus, mitochondria, and plasma membrane.

 

 

Service

  • Habitat for Humanity, Erie, PA Affiliate; Family Selection Committee (2009- present) ; Chair, Family Selection Committee (2010 - 2013; 2015 - present); Board Member (2013 - present); Board Secretary (2015-present)
  • Crime Victim Center, Erie PA, Board Member (2012-2015)